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验证数据展示

  • IF Staining of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

    IF Staining of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS18 antibody (83327-1-RR, Clone: 240004C10 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83327-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

    FC experiment of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPS18 Recombinant antibody (83327-1-RR, Clone:240004C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83327-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83327-1-RR

    Affinity and Kinetic Characterization of 83327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human RPS18 were performed. The affinity constant is 0.47 nM.

RPS18 Recombinant monoclonal antibody, PBS Only

RPS18 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83327-1-PBS

产品说明书

CloneNo. 240004C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

240004C10, D6S218E, HKE3, KE 3, KE3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83327-1-PBS targets RPS18 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12505

Product name: Recombinant human RPS18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC106063

Sequence: MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S18
别名 240004C10, D6S218E, HKE3, KE 3, KE3
计算分子量 152 aa, 18 kDa
GenBank蛋白编号BC106063
基因名称 RPS18
Gene ID (NCBI) 6222
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP62269
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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