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验证数据展示

  • WB analysis using 83848-4-RR

    WB analysis using 83848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83848-4-RR

    IF Staining of U2OS using 83848-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83848-4-RR

    FC experiment of HeLa using 83848-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83848-4-RR

    Affinity and Kinetic Characterization of 83848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

RPS17 Recombinant antibody

RPS17 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83848-4-RR

产品说明书

CloneNo. 240851H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83848-4-RR targets RPS17 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RPS17 fusion protein Ag9334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S17
别名 RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17
计算分子量 135 aa, 16 kDa
观测分子量16 kDa
GenBank蛋白编号BC009407
基因名称 RPS17
Gene ID (NCBI) 6218
RRIDAB_3671434
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08708
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS17 antibody 83848-4-RRDownload protocol
IF protocol for RPS17 antibody 83848-4-RRDownload protocol
FC protocol for RPS17 antibody 83848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols