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Host
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 83848-4-RR (same clone as 83848-4-PBS)

    WB analysis using 83848-4-RR (same clone as 83848-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83848-4-RR (same clone as 83848-4-PBS)

    IF Staining of U2OS using 83848-4-RR (same clone as 83848-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83848-4-RR (same clone as 83848-4-PBS)

    FC experiment of HeLa using 83848-4-RR (same clone as 83848-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00785-2

    Cytometric bead array standard curve of MP00785-2

    Cytometric bead array standard curve of MP00785-2, RPS17 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83848-4-PBS. Detection antibody: 83848-3-PBS. Standard: Ag9334. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83848-4-RR

    Affinity and Kinetic Characterization of 83848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

RPS17 Recombinant antibody, PBS Only (Capture)

RPS17 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83848-4-PBS

产品说明书

CloneNo. 240851H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83848-4-PBS targets RPS17 as part of a matched antibody pair:

MP00785-2: 83848-4-PBS capture and 83848-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RPS17 fusion protein Ag9334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S17
别名 RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17
计算分子量 135 aa, 16 kDa
观测分子量16 kDa
GenBank蛋白编号BC009407
基因名称 RPS17
Gene ID (NCBI) 6218
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08708
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.