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验证数据展示

  • WB analysis using 24542-1-AP (same clone as 24542-1-PBS)

    WB analysis using 24542-1-AP (same clone as 24542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24542-1-AP (same clone as 24542-1-PBS)

    IF Staining of HeLa using 24542-1-AP (same clone as 24542-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24542-1-AP (RPRD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 24542-1-AP (same clone as 24542-1-PBS)

    IHC staining of human stomach cancer using 24542-1-AP (same clone as 24542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24542-1-AP (RPRD1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPRD1B antibody included
  • IHC staining of human cervical cancer using 24542-1-AP (same clone as 24542-1-PBS)

    IHC staining of human cervical cancer using 24542-1-AP (same clone as 24542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24542-1-AP (RPRD1B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPRD1B antibody included
  • Featured Product
  • KD/KO Validated

RPRD1B Polyclonal antibody, PBS Only

RPRD1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24542-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C20orf77, Cell cycle-related and expression-elevated protein in tumor, CREPT, Regulation of nuclear pre-mRNA domain-containing protein 1B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPRD1B Ready-To-Use IHC Kit. RPRD1B primary antibody included.

产品信息

24542-1-PBS targets RPRD1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21578

Product name: Recombinant human RPRD1B protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 79-181 aa of BC033629

Sequence: REFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulation of nuclear pre-mRNA domain containing 1B
别名 C20orf77, Cell cycle-related and expression-elevated protein in tumor, CREPT, Regulation of nuclear pre-mRNA domain-containing protein 1B
计算分子量 326 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC033629
基因名称 RPRD1B
Gene ID (NCBI) 58490
RRIDAB_2879597
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQG5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPRD1B, also known as CREPT, is a protein-coding gene located on chromosome 20q11.23 that encodes a 326-amino-acid nuclear protein. RPRD1B functions primarily as a scaffold for the C-terminal domain (CTD) of RNA polymerase II (RNAPII), interacting with phosphorylated CTD repeats via its CTD-interaction domain (CID) and coordinating the dephosphorylation of phospho-Ser5 by the RPAP2 phosphatase. It forms homodimers and heterodimers with its paralog RPRD1A through coiled-coil domains and participates in transcription-coupled RNA processing and RNAPII promoter clearance. Functionally, RPRD1B enhances the expression of cell cycle-related genes, notably cyclin D1 (CCND1), by promoting RNAPII binding to the CCND1 promoter and facilitating loop formation that may enable RNAPII recycling during transcription. Beyond its physiological roles in transcription regulation and cell proliferation, RPRD1B is frequently overexpressed across multiple cancer types and has been shown to promote tumorigenesis by accelerating cell cycle progression, enhancing proliferative signaling, and supporting cancer cell migration and invasion, positioning it as a promising diagnostic and prognostic biomarker.

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