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RPRD1B Polyclonal antibody

RPRD1B Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24542-1-AP

Print datasheet

Synonyms

C20orf77, CREPT, RPRD1B

验证数据展示

  • WB analysis of HeLa using 24542-1-AP

    WB analysis of HeLa using 24542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24542-1-AP

    IF Staining of HeLa using 24542-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24542-1-AP (RPRD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24542-1-AP is RPRD1B Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RPRD1B fusion protein Ag21578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulation of nuclear pre-mRNA domain containing 1B
Synonyms C20orf77, CREPT, RPRD1B
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC033629
Gene Symbol RPRD1B
Gene ID (NCBI) 58490
RRIDAB_2879597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQG5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPRD1B antibody 24542-1-APDownload protocol
IF protocol for RPRD1B antibody 24542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PINK1-deficient Human Dopamine Neurons

Authors - Hu Wang
  • KO Validated
View Article