RPN1 Polyclonal antibody
RPN1 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells |
Positive IHC detected in | mouse lung tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 9 publications below |
IHC | See 1 publications below |
产品信息
12894-1-AP targets RPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, WB,ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, yeast |
Immunogen | RPN1 fusion protein Ag3576 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribophorin I |
Synonyms | hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1 |
Calculated Molecular Weight | 69 kDa |
Observed Molecular Weight | 66-69 kDa |
GenBank Accession Number | BC010839 |
Gene Symbol | RPN1 |
Gene ID (NCBI) | 6184 |
RRID | AB_2170474 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04843 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RPN1 antibody 12894-1-AP | Download protocol |
IHC protocol for RPN1 antibody 12894-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. | ||
Int J Mol Sci Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats. | ||
Proteomics Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action. |