验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RPL7A Ready-To-Use IHC Kit. RPL7A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15340-1-AP targets RPL7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPL7A fusion protein Ag7543 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L7a |
| 别名 | SURF 3, ribosomal protein L7a, PLA-X polypeptide, PLA X polypeptide, Large ribosomal subunit protein eL8 |
| 计算分子量 | 266 aa, 30 kDa |
| 观测分子量 | 30-32 kDa |
| GenBank蛋白编号 | BC005128 |
| 基因名称 | RPL7A |
| Gene ID (NCBI) | 6130 |
| RRID | AB_2254051 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62424 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL7A antibody 15340-1-AP | Download protocol |
| IHC protocol for RPL7A antibody 15340-1-AP | Download protocol |
| IF protocol for RPL7A antibody 15340-1-AP | Download protocol |
| IP protocol for RPL7A antibody 15340-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. | ||
Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Nucleic Acids Res The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis | ||
Am J Cancer Res UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc | ||
Nat Commun Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction |
