• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67729-1-Ig

    WB analysis using 67729-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67729-1-Ig (RPL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 67729-1-Ig

    IF Staining of L02 using 67729-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using RPL6 antibody (67729-1-Ig, Clone: 3F6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

RPL6 Monoclonal antibody

RPL6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67729-1-Ig

产品说明书

CloneNo. 3F6F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

3F6F8, 60S ribosomal protein L6, Large ribosomal subunit protein eL6, Neoplasm related protein C140, Neoplasm-related protein C140

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy RPL6 Ready-To-Use IHC Kit. RPL6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67729-1-Ig targets RPL6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL6 fusion protein Ag6840 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein L6
别名 3F6F8, 60S ribosomal protein L6, Large ribosomal subunit protein eL6, Neoplasm related protein C140, Neoplasm-related protein C140
计算分子量 33 kDa
观测分子量34 kDa
GenBank蛋白编号BC004138
基因名称 RPL6
Gene ID (NCBI) 6128
RRIDAB_2882915
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02878
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].

实验方案

Product Specific Protocols
WB protocol for RPL6 antibody 67729-1-IgDownload protocol
IF protocol for RPL6 antibody 67729-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols