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验证数据展示

  • WB analysis using 85348-1-RR

    WB analysis using 85348-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85348-1-RR (RPL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85348-1-RR

    IF Staining of HeLa using 85348-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPL5 antibody (85348-1-RR, Clone: 242678D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RPL5 Recombinant monoclonal antibody

RPL5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85348-1-RR

产品说明书

CloneNo. 242678D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, monkey

应用

WB, IF/ICC, ELISA

别名

60S ribosomal protein L5, DBA6, Large ribosomal subunit protein uL18, MSTP030, ribosomal protein L5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, 3T3-L1 cells, A431 cells, HeLa cells, Jurkat cells, MOLT-4 cells, COS-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® RPL5 Ready-To-Use IHC Kit. RPL5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85348-1-RR targets RPL5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, monkey
免疫原

CatNo: Ag30837

Product name: Recombinant human RPL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-297 aa of BC109370

Sequence: KRFPGYDSESKEFNAEVHRKHIMGQNVADYMRCLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L5
别名 60S ribosomal protein L5, DBA6, Large ribosomal subunit protein uL18, MSTP030, ribosomal protein L5
观测分子量 34 kDa
GenBank蛋白编号BC109370
基因名称 RPL5
Gene ID (NCBI) 6125
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP46777
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL5 (Ribosomal Protein L5) is a crucial component of the 60S ribosomal subunit, playing a significant role in ribosome biogenesis and function. It is required for rRNA maturation and the formation of the 60S ribosomal subunits. Defects or mutations in RPL5 are associated with Diamond-Blackfan anemia (DBA), a congenital disorder characterized by red blood cell aplasia.(PMID: 28376382)

实验方案

Product Specific Protocols
IF protocol for RPL5 antibody 85348-1-RRDownload protocol
WB protocol for RPL5 antibody 85348-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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