验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, 3T3-L1 cells, A431 cells, HeLa cells, Jurkat cells, MOLT-4 cells, COS-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85348-1-RR targets RPL5 in WB, ELISA applications and shows reactivity with human, mouse, monkey samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, monkey |
| 免疫原 | RPL5 fusion protein Ag30837 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L5 |
| 别名 | 60S ribosomal protein L5, DBA6, Large ribosomal subunit protein uL18, MSTP030, ribosomal protein L5 |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC109370 |
| 基因名称 | RPL5 |
| Gene ID (NCBI) | 6125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46777 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPL5 (Ribosomal Protein L5) is a crucial component of the 60S ribosomal subunit, playing a significant role in ribosome biogenesis and function. It is required for rRNA maturation and the formation of the 60S ribosomal subunits. Defects or mutations in RPL5 are associated with Diamond-Blackfan anemia (DBA), a congenital disorder characterized by red blood cell aplasia.(PMID: 28376382)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL5 antibody 85348-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
