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验证数据展示

  • WB analysis of mouse heart using 14826-1-AP

    WB analysis of mouse heart using 14826-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of HeLa using 14826-1-AP

    IF Staining of HeLa using 14826-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 14826-1-AP

    IHC staining of human liver cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human liver cancer using 14826-1-AP

    IHC staining of human liver cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human pancreas cancer using 14826-1-AP

    IHC staining of human pancreas cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human pancreas cancer using 14826-1-AP

    IHC staining of human pancreas cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of human stomach cancer using 14826-1-AP

    IHC staining of human stomach cancer using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of mouse pancreas using 14826-1-AP

    IHC staining of mouse pancreas using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included
  • IHC staining of rat pancreas using 14826-1-AP

    IHC staining of rat pancreas using 14826-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL35 antibody included

RPL35 Polyclonal antibody

RPL35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14826-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

ribosomal protein L35, Large ribosomal subunit protein uL29, 60S ribosomal protein L35

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected inrat pancreas tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPL35 Ready-To-Use IHC Kit. RPL35 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14826-1-AP targets RPL35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse, monkey
免疫原

CatNo: Ag6615

Product name: Recombinant human RPL35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-123 aa of BC000348

Sequence: MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L35
别名 ribosomal protein L35, Large ribosomal subunit protein uL29, 60S ribosomal protein L35
计算分子量 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC000348
基因名称 RPL35
Gene ID (NCBI) 11224
RRIDAB_2878083
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42766
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RPL35 antibody 14826-1-APDownload protocol
IHC protocol for RPL35 antibody 14826-1-APDownload protocol
WB protocol for RPL35 antibody 14826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Sci

Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.

Authors - Qiancong Zhao
View Article
monkeyWB

Zool Res

Ribosome-associated pathological TDP-43 alters the expression of multiple mRNAs in the monkey brain

Authors - Fu-Yu Deng
View Article
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