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RPL31 Polyclonal antibody

RPL31 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16497-1-AP

Print datasheet

Synonyms

60S ribosomal protein L31, ribosomal protein L31, RPL31

验证数据展示

  • WB analysis of HepG2 using 16497-1-AP

    WB analysis of HepG2 using 16497-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16497-1-AP

    IF Staining of HepG2 using 16497-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16497-1-AP (RPL31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, NIH/3T3 cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL31 Ready-To-Use IHC Kit. RPL31 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16497-1-AP targets RPL31 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPL31 fusion protein Ag9648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L31
Synonyms 60S ribosomal protein L31, ribosomal protein L31, RPL31
Calculated Molecular Weight 125 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC017343
Gene Symbol RPL31
Gene ID (NCBI) 6160
RRIDAB_2181772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62899
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center [PMID:22714919].

实验方案

Product Specific Protocols
WB protocol for RPL31 antibody 16497-1-APDownload protocol
IF protocol for RPL31 antibody 16497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
View Article
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk
View Article