验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66130 targets RPL3 in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag20400 Product name: Recombinant human RPL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 153-403 aa of BC012786 Sequence: MKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L3 |
| 别名 | 60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B |
| 计算分子量 | 46/27 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC012786 |
| 基因名称 | RPL3 |
| Gene ID (NCBI) | 6122 |
| RRID | AB_2919951 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P39023 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 RPL3 antibody CL594-66130 | Download protocol |
| WB protocol for CL594 RPL3 antibody CL594-66130 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
