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验证数据展示

  • WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

    WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

    FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83377-1-RR

    Affinity and Kinetic Characterization of 83377-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83377-1-RR against Human RPL29 were performed. The affinity constant is 0.254 nM.

RPL29 Recombinant monoclonal antibody, PBS Only

RPL29 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83377-1-PBS

产品说明书

CloneNo. 240062F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

240062F9, 60S ribosomal protein L29, Cell surface heparin-binding protein HIP, HIP, HUMRPL29

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83377-1-PBS targets RPL29 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34100

Product name: Recombinant human RPL29 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-97 aa of BC008926

Sequence: MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L29
别名 240062F9, 60S ribosomal protein L29, Cell surface heparin-binding protein HIP, HIP, HUMRPL29
计算分子量 159 aa, 18 kDa
GenBank蛋白编号BC008926
基因名称 RPL29
Gene ID (NCBI) 6159
RRIDAB_3671030
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP47914
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).

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