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RPL28 Polyclonal antibody

RPL28 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16649-1-AP

Print datasheet

Synonyms

60S ribosomal protein L28, ribosomal protein L28, RPL28

验证数据展示

  • WB analysis of HeLa using 16649-1-AP

    WB analysis of HeLa using 16649-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16649-1-AP (RPL28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16649-1-AP

    IF Staining of HepG2 using 16649-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPL28 antibody 16649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16649-1-AP targets RPL28 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB, IF, FC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL28 fusion protein Ag10036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L28
Synonyms 60S ribosomal protein L28, ribosomal protein L28, RPL28
Calculated Molecular Weight 137 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC010173
Gene Symbol RPL28
Gene ID (NCBI) 6158
RRIDAB_2254021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46779
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL28 antibody 16649-1-APDownload protocol
IF protocol for RPL28 antibody 16649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genes Dev

Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.

Authors - Andrew J Finch
View Article
humanFC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
View Article
humanWB

Aging Cell

Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence.

Authors - Asimina Pantazi
View Article
mouseWB

Elife

Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts

Authors - Jasbir Singh Dalal
View Article
mouseWB,IF

iScience

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

Authors - Qianxing Zou
View Article

Sci Rep

Germline variability and tumor expression level of ribosomal protein gene RPL28 are associated with survival of metastatic colorectal cancer patients.

Authors - Adrien Labriet
View Article