验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14980-1-AP targets RPL27 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6893 Product name: Recombinant human RPL27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-136 aa of BC001700 Sequence: MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L27 |
| 别名 | 60S ribosomal protein L27, ribosomal protein L27, RPL27 |
| 计算分子量 | 16 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC001700 |
| 基因名称 | RPL27 |
| Gene ID (NCBI) | 6155 |
| RRID | AB_2181742 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61353 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPL27 antibody 14980-1-AP | Download protocol |
| WB protocol for RPL27 antibody 14980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Orthop Translat Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression. | ||
Physiol Rep Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules. | ||
EMBO Rep miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity. | ||
Brain Sci Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest | ||
Nat Struct Mol Biol Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment |
