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验证数据展示

  • WB analysis using 86520-1-RR (same clone as 86520-1-PBS)

    WB analysis using 86520-1-RR (same clone as 86520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86520-1-RR (RPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 86520-1-RR (same clone as 86520-1-PBS)

    IHC staining of human tonsillitis using 86520-1-RR (same clone as 86520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86520-1-RR (RPL23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL23 antibody included
  • IF Staining of HeLa using 86520-1-RR (same clone as 86520-1-PBS)

    IF Staining of HeLa using 86520-1-RR (same clone as 86520-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL23 antibody (86520-1-RR, Clone: 251372C7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

RPL23 Recombinant monoclonal antibody, PBS Only

RPL23 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86520-1-PBS

产品说明书

CloneNo. 251372C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Large ribosomal subunit protein uL14, ribosomal protein L23, rpL17

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPL23 Ready-To-Use IHC Kit. RPL23 primary antibody included.

产品信息

86520-1-PBS targets RPL23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9120

Product name: Recombinant human RPL23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-140 aa of BC010114

Sequence: MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L23
别名 Large ribosomal subunit protein uL14, ribosomal protein L23, rpL17
计算分子量 140 aa, 15 kDa
观测分子量15 kDa
GenBank蛋白编号BC010114
基因名称 RPL23
Gene ID (NCBI) 9349
RRIDAB_3744942
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62829
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].

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