验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse spleen tissue, mouse testis tissue, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
14701-1-AP targets RPL19 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6371 Product name: Recombinant human RPL19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-196 aa of BC066315 Sequence: MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L19 |
| 别名 | 60S ribosomal protein L19, Large ribosomal subunit protein eL19, ribosomal protein L19 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC066315 |
| 基因名称 | RPL19 |
| Gene ID (NCBI) | 6143 |
| RRID | AB_2181587 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P84098 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL19 antibody 14701-1-AP | Download protocol |
| IHC protocol for RPL19 antibody 14701-1-AP | Download protocol |
| IP protocol for RPL19 antibody 14701-1-AP | Download protocol |
| WB protocol for RPL19 antibody 14701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. | ||
Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. | ||
EMBO J Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling. | ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
Hum Mutat Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia. | ||
EMBO J Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling. |
