验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67223-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67223 targets RPL17 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | RPL17 fusion protein Ag5393 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L17 |
| 别名 | 60S ribosomal protein L17, 60S ribosomal protein L23, FLJ92089, PD 1, ribosomal protein L17, RPL17, rpL23 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC066323 |
| 基因名称 | RPL17 |
| Gene ID (NCBI) | 6139 |
| RRID | AB_3084328 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P18621 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RPL17 antibody CL488-67223 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |