验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, A2780 cells, LNCap cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68546-1-Ig targets RPAIN in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | RPAIN fusion protein Ag7844 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RPA interacting protein |
| 别名 | HRIP, RIP, RPA interacting protein, RPAIN |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC004451 |
| 基因名称 | RPAIN |
| Gene ID (NCBI) | 84268 |
| RRID | AB_3085251 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q86UA6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. RPAIN was originally identified as a nuclear transporter of RPA in Xenopus (PMID: 23010595). The human RIPα gene encodes several splice isoforms, of which hRIPα and hRIPβ are the major translation products in vivo.hRIPα transports RPA into the nucleus by shuttling between the cytoplasm and nucleus while hRIPβ is localized in promyelocytic leukemia (PML) nuclear bodies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPAIN antibody 68546-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
