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验证数据展示

  • WB analysis of A2780 using 15220-1-AP (same clone as 15220-1-PBS)

    WB analysis of A2780 using 15220-1-AP (same clone as 15220-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • IP experiment of A375 using 15220-1-AP (same clone as 15220-1-PBS)

    IP experiment of A375 using 15220-1-AP (same clone as 15220-1-PBS)

    IP result of anti-RPAIN (IP:15220-1-AP, 4ug; Detection:15220-1-AP 1:300) with A375 cells lysate 2200 ug. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • WB analysis of A375 using 15220-1-AP (same clone as 15220-1-PBS)

    WB analysis of A375 using 15220-1-AP (same clone as 15220-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

    IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

    IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

RPAIN Polyclonal antibody, PBS Only

RPAIN Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15220-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15220-1-PBS targets RPAIN in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7392

Product name: Recombinant human RPAIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-145 aa of BC004451

Sequence: MAESLRSPRRSLYKLVGSPPWKEAFRQRCLERMRNSRDRLLNRYRQAGSSGPGNSQNSFLVQEVMEEEWNALQSVENCPEDLAQLEELIDMAVLEEIQQELINQEQSIISEYEKSLQFDEKCLSIMLAEWEANPLICPVCTNLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RPA interacting protein
别名
计算分子量 25 kDa
观测分子量 30-45 kDa
GenBank蛋白编号BC004451
基因名称 RPAIN
Gene ID (NCBI) 84268
RRIDAB_2180976
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UA6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.

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