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验证数据展示

  • WB analysis of A2780 using 15220-1-AP

    WB analysis of A2780 using 15220-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A375 using 15220-1-AP

    IP experiment of A375 using 15220-1-AP

    IP result of anti-RPAIN (IP:15220-1-AP, 4ug; Detection:15220-1-AP 1:300) with A375 cells lysate 2200 ug.

  • WB analysis of A375 using 15220-1-AP

    WB analysis of A375 using 15220-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15220-1-AP

    IHC staining of human ovary tumor using 15220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 15220-1-AP

    IHC staining of human ovary tumor using 15220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 40x lens).

RPAIN Polyclonal antibody

RPAIN Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15220-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

HRIP, RIP, RPA interacting protein, RPAIN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, mouse ovary tissue, A2780 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15220-1-AP targets RPAIN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7392

Product name: Recombinant human RPAIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-145 aa of BC004451

Sequence: MAESLRSPRRSLYKLVGSPPWKEAFRQRCLERMRNSRDRLLNRYRQAGSSGPGNSQNSFLVQEVMEEEWNALQSVENCPEDLAQLEELIDMAVLEEIQQELINQEQSIISEYEKSLQFDEKCLSIMLAEWEANPLICPVCTNLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RPA interacting protein
别名 HRIP, RIP, RPA interacting protein, RPAIN
计算分子量 25 kDa
观测分子量 30-45 kDa
GenBank蛋白编号BC004451
基因名称 RPAIN
Gene ID (NCBI) 84268
RRIDAB_2180976
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.

实验方案

Product Specific Protocols
IHC protocol for RPAIN antibody 15220-1-APDownload protocol
IP protocol for RPAIN antibody 15220-1-APDownload protocol
WB protocol for RPAIN antibody 15220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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