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验证数据展示

  • WB analysis using 87254-1-RR

    WB analysis using 87254-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87254-1-RR (RPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 87254-1-RR

    IF Staining of A431 using 87254-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (87254-1-RR, Clone: 252472D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 87254-1-RR

    Affinity of 87254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87254-1-RR against Human RPA were performed. The affinity constant is 1.22 nM.

RPA2 Recombinant monoclonal antibody

RPA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87254-1-RR

产品说明书

CloneNo. 252472D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat testis tissue, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, THP-1 cells, mouse testis tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87254-1-RR targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0690

Product name: Recombinant human RPA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-270 aa of BC001630

Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 replication protein A2, 32kDa
别名 REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC001630
基因名称 RPA2
Gene ID (NCBI) 6118
RRIDAB_3745434
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15927
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

实验方案

Product Specific Protocols
IF protocol for RPA2 antibody 87254-1-RRDownload protocol
WB protocol for RPA2 antibody 87254-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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