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验证数据展示

  • WB analysis using 87254-1-RR (same clone as 87254-1-PBS)

    WB analysis using 87254-1-RR (same clone as 87254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87254-1-RR (RPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87254-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 87254-1-RR (same clone as 87254-1-PBS)

    IF Staining of A431 using 87254-1-RR (same clone as 87254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (87254-1-RR, Clone: 252472D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87254-1-PBS in a different storage buffer formulation.

  • Affinity of 87254-1-RR

    Affinity of 87254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87254-1-RR against Human RPA were performed. The affinity constant is 1.22 nM.

RPA2 Recombinant monoclonal antibody, PBS Only

RPA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87254-1-PBS

产品说明书

CloneNo. 252472D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87254-1-PBS targets RPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0690

Product name: Recombinant human RPA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-270 aa of BC001630

Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 replication protein A2, 32kDa
别名 REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC001630
基因名称 RPA2
Gene ID (NCBI) 6118
RRIDAB_3745434
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15927
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

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