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验证数据展示

  • WB analysis using 82930-1-RR (same clone as 82930-1-PBS)

    WB analysis using 82930-1-RR (same clone as 82930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82930-1-RR (RORA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 82930-1-PBS

    IHC staining of rat cerebellum using 82930-1-PBS

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-PBS (RORA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 82930-1-PBS

    IF Staining of HepG2 using 82930-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-PBS, Clone: 230217G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of rat cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

    IHC staining of rat cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 82930-1-RR (same clone as 82930-1-PBS)

    IF Staining of rat brain using 82930-1-RR (same clone as 82930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82930-1-RR (same clone as 82930-1-PBS)

    FC experiment of HeLa using 82930-1-RR (same clone as 82930-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RORA Recombinant antibody (82930-1-RR, Clone:230217G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82930-1-PBS

    FC experiment of HeLa using 82930-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RORA Recombinant antibody, PBS Only (82930-1-PBS, Clone:230217G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

    IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

    IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

RORA Recombinant antibody, PBS Only

RORA Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 82930-1-PBS

产品说明书

CloneNo. 230217G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

230217G6, NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82930-1-PBS targets RORA in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RORA fusion protein Ag0944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAR-related orphan receptor A
别名 230217G6, NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A
计算分子量 59 kDa
观测分子量60-66 kDa
GenBank蛋白编号BC008831
基因名称 RORA
Gene ID (NCBI) 6095
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35398
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

实验方案

Product Specific Protocols
IHC protocol for RORA antibody 82930-1-PBSDownload protocol
IF protocol for RORA antibody 82930-1-PBSDownload protocol
FC protocol for RORA antibody 82930-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols