验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20629-1-AP targets ROR1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | ROR1 fusion protein Ag14586 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | receptor tyrosine kinase-like orphan receptor 1 |
| 别名 | NTRKR1, ROR1 |
| 计算分子量 | 937 aa, 104 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | BC006374 |
| 基因名称 | ROR1 |
| Gene ID (NCBI) | 4919 |
| RRID | AB_10694571 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01973 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROR1 is a member of the RTK family of orphan receptors related to muscle specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937-amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROR1 antibody 20629-1-AP | Download protocol |
| IHC protocol for ROR1 antibody 20629-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virchows Arch Expression of ROR1 has prognostic significance in triple negative breast cancer. | ||
Medicina (Kaunas) Meta-Analysis of Survival Effects of Receptor Tyrosine Kinase-like Orphan Receptor 1 (ROR1) | ||
Biomedicines ROR1 Is Expressed in Diffuse Large B-Cell Lymphoma (DLBCL) and a Small Molecule Inhibitor of ROR1 (KAN0441571C) Induced Apoptosis of Lymphoma Cells. | ||
Cancer Manag Res Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cells. | ||
Am J Physiol Cell Physiol Periodontal Ligaments Enhance Neurite Outgrowth in Trigeminal Ganglion Neurons through Wnt5a Production Induced by Mechanical Stimulation |
