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验证数据展示

  • WB analysis using 20220-1-AP

    WB analysis using 20220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20220-1-AP (ROBO3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 20220-1-AP

    IHC staining of human gliomas using 20220-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

  • IHC staining of human gliomas using 20220-1-AP

    IHC staining of human gliomas using 20220-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

ROBO3-Specific Polyclonal antibody

ROBO3-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20220-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

ROBO3, Roundabout-like protein 3, RIG1, RBIG1, HGPS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20220-1-AP targets ROBO3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 roundabout, axon guidance receptor, homolog 3 (Drosophila)
别名 ROBO3, Roundabout-like protein 3, RIG1, RBIG1, HGPS
计算分子量 148 kDa
观测分子量 148 kDa
GenBank蛋白编号NM_022370
基因名称 ROBO3
Gene ID (NCBI) 64221
RRIDAB_10732825
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. The antibody is specific to ROBO3.

实验方案

Product Specific Protocols
WB protocol for ROBO3-Specific antibody 20220-1-APDownload protocol
IHC protocol for ROBO3-Specific antibody 20220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Non-redundant roles of DIAPHs in primary ciliogenesis.

Authors - Oliva Palander
View Article
mouseWB

Brain Res Bull

CircSATB2 Modulates Fear Extinction Memory via Robo3-Driven Synaptic Plasticity

Authors - Ziyue Xu
View Article