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验证数据展示

  • WB analysis of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

    WB analysis of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

    IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

    IF Staining of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNMTL1 Polyclonal antibody, PBS Only

RNMTL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14707-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14707-1-PBS targets RNMTL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6399

Product name: Recombinant human RNMTL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC050614

Sequence: MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQREKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAVPKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGVMGIFAKPDHVKMTYPKTQLQHSLPLLLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPPDTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAPAAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA methyltransferase like 1
别名
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC050614
基因名称 RNMTL1
Gene ID (NCBI) 55178
RRIDAB_2181563
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC36
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].

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