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验证数据展示

  • WB analysis of HeLa using 14707-1-AP

    WB analysis of HeLa using 14707-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 14707-1-AP

    IHC staining of human liver using 14707-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14707-1-AP

    IF Staining of HeLa using 14707-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

RNMTL1 Polyclonal antibody

RNMTL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14707-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

RMTL1, MRM3, HC90, EC:2.1.1.-, 16S rRNA [Gm1370] 2'-O-methyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14707-1-AP targets RNMTL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag6399

Product name: Recombinant human RNMTL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC050614

Sequence: MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQREKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAVPKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGVMGIFAKPDHVKMTYPKTQLQHSLPLLLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPPDTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAPAAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA methyltransferase like 1
别名 RMTL1, MRM3, HC90, EC:2.1.1.-, 16S rRNA [Gm1370] 2'-O-methyltransferase
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC050614
基因名称 RNMTL1
Gene ID (NCBI) 55178
RRIDAB_2181563
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].

实验方案

Product Specific Protocols
WB protocol for RNMTL1 antibody 14707-1-APDownload protocol
IHC protocol for RNMTL1 antibody 14707-1-APDownload protocol
IF protocol for RNMTL1 antibody 14707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB,IF

J Biol Chem

Mitochondrial rRNA Methyltransferase Family Members are Positioned to Modify Nascent rRNA in Foci Near the mtDNA Nucleoid.

Authors - Lee Ken-Wing KW
  • KD Validated
View Article
HumanIHC

PeerJ

REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma.

Authors - Zhiqiang Yin
View Article