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验证数据展示

  • FC experiment of HepG2 using 83974-4-RR

    FC experiment of HepG2 using 83974-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-4-RR, Clone:240927H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IHC staining of human placenta using 83974-4-RR

    IHC staining of human placenta using 83974-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 83974-4-RR

    IHC staining of human placenta using 83974-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83974-4-RR

    IF Staining of HepG2 using 83974-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNGTT antibody (83974-4-RR, Clone: 240927H2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83974-4-RR

    Affinity and Kinetic Characterization of 83974-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-4-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

RNGTT Recombinant monoclonal antibody

RNGTT Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83974-4-RR

产品说明书

CloneNo. 240927H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83974-4-RR targets RNGTT in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3105

Product name: Recombinant human RNGTT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 242-598 aa of BC019954

Sequence: TQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPHPLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA guanylyltransferase and 5'-phosphatase
别名 hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A
计算分子量 597 aa, 69 kDa
GenBank蛋白编号BC019954
基因名称 RNGTT
Gene ID (NCBI) 8732
RRIDAB_3671550
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60942
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for RNGTT antibody 83974-4-RRDownload protocol
IHC protocol for RNGTT antibody 83974-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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