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验证数据展示

  • FC experiment of HepG2 using 83974-4-RR (same clone as 83974-4-PBS)

    FC experiment of HepG2 using 83974-4-RR (same clone as 83974-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-4-RR, Clone:240927H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83974-4-RR (same clone as 83974-4-PBS)

    IHC staining of human placenta using 83974-4-RR (same clone as 83974-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83974-4-RR (same clone as 83974-4-PBS)

    IHC staining of human placenta using 83974-4-RR (same clone as 83974-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83974-4-RR (same clone as 83974-4-PBS)

    IF Staining of HepG2 using 83974-4-RR (same clone as 83974-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNGTT antibody (83974-4-RR, Clone: 240927H2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83974-4-PBS

    Affinity and Kinetic Characterization of 83974-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83974-4-PBS against Human RNGTT was performed. The affinity constant is 0.319 nM.

  • Affinity and Kinetic Characterization of 83974-4-RR

    Affinity and Kinetic Characterization of 83974-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-4-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

RNGTT Recombinant monoclonal antibody, PBS Only

RNGTT Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83974-4-PBS

产品说明书

CloneNo. 240927H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

240927H2, CAP1A, GTase, GTP  RNA guanylyltransferase, GTP--RNA guanylyltransferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83974-4-PBS targets RNGTT in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3105

Product name: Recombinant human RNGTT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 242-598 aa of BC019954

Sequence: TQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPHPLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA guanylyltransferase and 5'-phosphatase
别名 240927H2, CAP1A, GTase, GTP  RNA guanylyltransferase, GTP--RNA guanylyltransferase
计算分子量 597 aa, 69 kDa
GenBank蛋白编号BC019954
基因名称 RNGTT
Gene ID (NCBI) 8732
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60942
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

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