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验证数据展示

  • WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84058-4-RR

    Affinity and Kinetic Characterization of 84058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-4-RR against Human RNF40 were performed. The affinity constant is 1.34 nM.

RNF40 Recombinant monoclonal antibody, PBS Only

RNF40 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84058-4-PBS

产品说明书

CloneNo. 241214D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84058-4-PBS targets RNF40 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8047

Product name: Recombinant human RNF40 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 700-1001 aa of BC006133

Sequence: VDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 40
别名 E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein
计算分子量 114 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC006133
基因名称 RNF40
Gene ID (NCBI) 9810
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO75150
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

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