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验证数据展示

  • WB analysis using 30797-1-AP (same clone as 30797-1-PBS)

    WB analysis using 30797-1-AP (same clone as 30797-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 30797-1-AP (same clone as 30797-1-PBS)

    WB analysis of HEK-293 using 30797-1-AP (same clone as 30797-1-PBS)

    WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 30797-1-AP (same clone as 30797-1-PBS)

    IF Staining of HepG2 using 30797-1-AP (same clone as 30797-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNF31 / HOIP Polyclonal antibody, PBS Only

RNF31 / HOIP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30797-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30797-1-PBS targets RNF31 / HOIP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34505

Product name: Recombinant human RNF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-603 aa of BC017376

Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 31
别名
计算分子量 1072 aa, 120 kDa
观测分子量 100 kDa
GenBank蛋白编号BC017376
基因名称 RNF31
Gene ID (NCBI) 55072
RRIDAB_3086418
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EP0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)

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