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验证数据展示

  • WB analysis using 30797-1-AP

    WB analysis using 30797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 30797-1-AP

    WB analysis of HEK-293 using 30797-1-AP

    WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells.

  • IF Staining of HepG2 using 30797-1-AP

    IF Staining of HepG2 using 30797-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

RNF31 / HOIP Polyclonal antibody

RNF31 / HOIP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30797-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, Raji cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30797-1-AP targets RNF31 / HOIP in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag34505

Product name: Recombinant human RNF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-603 aa of BC017376

Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 31
别名 FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA
计算分子量 1072 aa, 120 kDa
观测分子量 100 kDa
GenBank蛋白编号BC017376
基因名称 RNF31
Gene ID (NCBI) 55072
RRIDAB_3086418
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF31 / HOIP antibody 30797-1-APDownload protocol
IF protocol for RNF31 / HOIP antibody 30797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols