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RNF168 Polyclonal antibody

RNF168 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 21393-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA and More (2)

RING-type E3 ubiquitin transferase RNF168, RING finger protein 168, hRNF168, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF168

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, human brain tissue, Jurkat cells, Transfected HEK-293 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21393-1-AP targets RNF168 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP, CoIP
经测试反应性 human
文献引用反应性human
免疫原 RNF168 fusion protein Ag16055 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 168
别名 RING-type E3 ubiquitin transferase RNF168, RING finger protein 168, hRNF168, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF168
计算分子量 571 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC033791
基因名称 RNF168
Gene ID (NCBI) 165918
RRIDAB_10733883
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF168 antibody 21393-1-APDownload protocol
IF protocol for RNF168 antibody 21393-1-APDownload protocol
IP protocol for RNF168 antibody 21393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

Authors - Kaiwen Bao
humanIP

Nat Commun

Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.

Authors - Qinglei Hang
human

Nucleic Acids Res

GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.

Authors - Xiaopeng Lu
humanWB

Theranostics

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

Authors - Qi Zhang
humanWB,IF

J Cell Biol

DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response

Authors - Tessa Arends
humanWB

EMBO Rep

An LTR retrotransposon-derived lncRNA interacts with RNF169 to promote homologous recombination.

Authors - Bing Deng
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