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验证数据展示

  • WB analysis of HeLa using 26306-1-AP

    WB analysis of HeLa using 26306-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26306-1-AP

    IP experiment of HeLa using 26306-1-AP

    IP result of anti-RNF144B/IBRDC2 (IP:26306-1-AP, 4ug; Detection:26306-1-AP 1:300) with HeLa cells lysate 2400ug.

  • IHC staining of human kidney using 26306-1-AP

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RNF144B/IBRDC2 antibody included
  • IHC staining of human kidney using 26306-1-AP

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RNF144B/IBRDC2 antibody included
  • IHC staining of mouse kidney using 26306-1-AP

    IHC staining of mouse kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF144B/IBRDC2 antibody included
  • IHC staining of human placenta using 26306-1-AP

    IHC staining of human placenta using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF144B/IBRDC2 antibody included
  • IHC staining of mouse adrenal gland using 26306-1-AP

    IHC staining of mouse adrenal gland using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF144B/IBRDC2 antibody included
  • IHC staining of rat adrenal gland using 26306-1-AP

    IHC staining of rat adrenal gland using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF144B/IBRDC2 antibody included

RNF144B/IBRDC2 Polyclonal antibody

RNF144B/IBRDC2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26306-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA and More (1)

别名

IBRDC2, RNF144B, E3 ubiquitin-protein ligase RNF144B, EC:2.3.2.31, IBR domain-containing protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, mouse kidney tissue, rat adrenal gland tissue, mouse adrenal gland tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® RNF144B/IBRDC2 Ready-To-Use IHC Kit. RNF144B/IBRDC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26306-1-AP targets RNF144B/IBRDC2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IP, CoIP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag23679

Product name: Recombinant human RNF144B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-187 aa of BC063311

Sequence: VADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 144B
别名 IBRDC2, RNF144B, E3 ubiquitin-protein ligase RNF144B, EC:2.3.2.31, IBR domain-containing protein 2
观测分子量 34 kDa
GenBank蛋白编号BC063311
基因名称 RNF144B
Gene ID (NCBI) 255488
RRIDAB_2880473
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z419
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IP protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
WB protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP

EMBO Rep

RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

Authors - Guoxiu Li
View Article
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