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验证数据展示

  • WB analysis of HeLa using 26306-1-AP

    WB analysis of HeLa using 26306-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26306-1-AP

    IP experiment of HeLa using 26306-1-AP

    IP result of anti-RNF144B/IBRDC2 (IP:26306-1-AP, 4ug; Detection:26306-1-AP 1:300) with HeLa cells lysate 2400ug.

  • IHC staining of human kidney using 26306-1-AP

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26306-1-AP

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 40x lens).

RNF144B/IBRDC2 Polyclonal antibody

RNF144B/IBRDC2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 26306-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA and More (1)

别名

IBRDC2, KIAA0161, p53RFP, ring finger protein 144B, RNF144B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26306-1-AP targets RNF144B/IBRDC2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP, CoIP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag23679

Product name: Recombinant human RNF144B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-187 aa of BC063311

Sequence: VADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 144B
别名 IBRDC2, KIAA0161, p53RFP, ring finger protein 144B, RNF144B
观测分子量 34 kDa
GenBank蛋白编号BC063311
基因名称 RNF144B
Gene ID (NCBI) 255488
RRIDAB_2880473
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z419
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IP protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
WB protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP

EMBO Rep

RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

Authors - Guoxiu Li
View Article
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