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RNF14 Polyclonal antibody

RNF14 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 26368-1-AP

Print datasheet

Synonyms

ARA54, HFB30, HRIHFB2038, ring finger protein 14, RNF14, TRIAD2, Triad2 protein

验证数据展示

  • WB analysis of HEK-293T using 26368-1-AP

    WB analysis of HEK-293T using 26368-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 26368-1-AP

    IP experiment of COLO 320 using 26368-1-AP

    IP result of anti-RNF14 (IP:26368-1-AP, 4ug; Detection:26368-1-AP 1:600) with COLO 320 cells lysate 4000ug.

  • IHC staining of human colon cancer using 26368-1-AP

    IHC staining of human colon cancer using 26368-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26368-1-AP (RNF14 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 26368-1-AP

    IF Staining of HEK-293 using 26368-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 26368-1-AP (RNF14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26368-1-AP is RNF14 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen RNF14 fusion protein Ag23734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 14
Synonyms ARA54, HFB30, HRIHFB2038, ring finger protein 14, RNF14, TRIAD2, Triad2 protein
Observed Molecular Weight 47-54 kDa
GenBank Accession NumberBC126185
Gene Symbol RNF14
Gene ID (NCBI) 9604
RRIDAB_2880494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF14 antibody 26368-1-APDownload protocol
IHC protocol for RNF14 antibody 26368-1-APDownload protocol
IF protocol for RNF14 antibody 26368-1-APDownload protocol
IP protocol for RNF14 antibody 26368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols