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RNF135 Polyclonal antibody

RNF135 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25061-1-AP

Print datasheet

Synonyms

L13, REUL, RIG I E3 ubiquitin ligase, ring finger protein 135, Riplet, RNF135

验证数据展示

  • WB analysis of mouse kidney using 25061-1-AP

    WB analysis of mouse kidney using 25061-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25061-1-AP

    IHC staining of human kidney using 25061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 25061-1-AP

    IHC staining of human kidney using 25061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of PC-12 using 25061-1-AP

    IF Staining of PC-12 using 25061-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using RNF135 antibody (25061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 25061-1-AP

    WB analysis using 25061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse kidney tissue, U-87 MG cells
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25061-1-AP targets RNF135 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RNF135 fusion protein Ag18821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 135
Synonyms L13, REUL, RIG I E3 ubiquitin ligase, ring finger protein 135, Riplet, RNF135
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC005084
Gene Symbol RNF135
Gene ID (NCBI) 84282
RRIDAB_2879879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.

实验方案

Product Specific Protocols
WB protocol for RNF135 antibody 25061-1-APDownload protocol
IHC protocol for RNF135 antibody 25061-1-APDownload protocol
IF protocol for RNF135 antibody 25061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination.

Authors - Yuan-Qin Min
View Article