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验证数据展示

  • WB analysis using 19835-1-AP

    WB analysis using 19835-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19835-1-AP (RNF126 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of LNCaP using 19835-1-AP

    IP experiment of LNCaP using 19835-1-AP

    IP result of anti-RNF126 (IP:19835-1-AP, 4ug; Detection:19835-1-AP 1:10000) with LNCaP cells lysate 880 ug.

  • IHC staining of mouse liver using 19835-1-AP

    IHC staining of mouse liver using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of human ovary cancer using 19835-1-AP

    IHC staining of human ovary cancer using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of human placenta using 19835-1-AP

    IHC staining of human placenta using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of human placenta using 19835-1-AP

    IHC staining of human placenta using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of mouse ovary using 19835-1-AP

    IHC staining of mouse ovary using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of mouse pancreas using 19835-1-AP

    IHC staining of mouse pancreas using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of rat liver using 19835-1-AP

    IHC staining of rat liver using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of rat ovary using 19835-1-AP

    IHC staining of rat ovary using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of rat pancreas using 19835-1-AP

    IHC staining of rat pancreas using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IHC staining of mouse ovary using 19835-1-AP

    IHC staining of mouse ovary using 19835-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19835-1-AP (RNF126 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RNF126 antibody included
  • IF Staining of A431 using 19835-1-AP

    IF Staining of A431 using 19835-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RNF126 antibody (19835-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RNF126 Polyclonal antibody

RNF126 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19835-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells, NIH/3T3 cells
Positive IP detected inLNCaP cells
Positive IHC detected inhuman placenta tissue, mouse liver tissue, human ovary cancer tissue, mouse ovary tissue, mouse pancreas tissue, rat liver tissue, rat ovary tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RNF126 Ready-To-Use IHC Kit. RNF126 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19835-1-AP targets RNF126 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag13816

Product name: Recombinant human RNF126 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-326 aa of BC001442

Sequence: MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQHLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRHEGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATNPPGLTGVSFSSSSSSSSSSSPSNENATWSPLGRPQPPRPLSNLTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 126
别名 E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126
计算分子量 326 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC001442
基因名称 RNF126
Gene ID (NCBI) 55658
RRIDAB_3085588
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

实验方案

Product Specific Protocols
IF protocol for RNF126 antibody 19835-1-APDownload protocol
IHC protocol for RNF126 antibody 19835-1-APDownload protocol
IP protocol for RNF126 antibody 19835-1-APDownload protocol
WB protocol for RNF126 antibody 19835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Med Chem

Design, Synthesis, and Biological Evaluation of a MyD88-Targeted Molecular Glue d21 for the Treatment of Acute Lung Injury.

Authors - Luxiao Zhu
View Article
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