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验证数据展示

  • WB analysis using 19835-1-AP

    WB analysis using 19835-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19835-1-AP (RNF126 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of LNCaP using 19835-1-AP

    IP experiment of LNCaP using 19835-1-AP

    IP result of anti-RNF126 (IP:19835-1-AP, 4ug; Detection:19835-1-AP 1:10000) with LNCaP cells lysate 880 ug.

  • IF Staining of A431 using 19835-1-AP

    IF Staining of A431 using 19835-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RNF126 antibody (19835-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RNF126 Polyclonal antibody

RNF126 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 19835-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells, NIH/3T3 cells
Positive IP detected inLNCaP cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RNF126 Ready-To-Use IHC Kit. RNF126 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19835-1-AP targets RNF126 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13816

Product name: Recombinant human RNF126 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-326 aa of BC001442

Sequence: MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQHLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRHEGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATNPPGLTGVSFSSSSSSSSSSSPSNENATWSPLGRPQPPRPLSNLTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 126
别名 E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126
计算分子量 326 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC001442
基因名称 RNF126
Gene ID (NCBI) 55658
RRIDAB_3085588
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

实验方案

Product Specific Protocols
IF protocol for RNF126 antibody 19835-1-APDownload protocol
IP protocol for RNF126 antibody 19835-1-APDownload protocol
WB protocol for RNF126 antibody 19835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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