验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66228-1-Ig targets RND3 in WB, IF, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag18839 Product name: Recombinant human RND3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-244 aa of BC012513 Sequence: MKERRASQKLSSKSIMDPNQNVKCKIVVVGDSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVATDLRKDKAKSCTVM 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Rho family GTPase 3 |
| 别名 | ARHE, memB, Protein MemB, Rho family GTPase 3, Rho8, RhoE, RND3 |
| 计算分子量 | 244 aa, 27 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC012513 |
| 基因名称 | RND3 |
| Gene ID (NCBI) | 390 |
| RRID | AB_2881619 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P61587 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RND3 antibody 66228-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE. | ||
Int J Mol Med Ingenuity pathway analysis of differentially expressed genes involved in signaling pathways and molecular networks in RhoE gene‑edited cardiomyocytes.
| ||
Virology Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection | ||
bioRxiv The Hexosamine Biosynthetic Pathway alters the cytoskeleton to modulate cell proliferation and migration in metastatic prostate cancer |
