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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68787-3-Ig (same clone as 68787-3-PBS)

    WB analysis using 68787-3-Ig (same clone as 68787-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68787-3-PBS in a different storage buffer formulation.

  • WB analysis using 68787-3-Ig (same clone as 68787-3-PBS)

    WB analysis using 68787-3-Ig (same clone as 68787-3-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68787-3-PBS in a different storage buffer formulation.

RNASEH1 Monoclonal antibody, PBS Only

RNASEH1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68787-3-PBS

产品说明书

CloneNo. 2C8D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C8D3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68787-3-PBS targets RNASEH1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33948

Product name: Recombinant human RNASEH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-286 aa of BC002973

Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribonuclease H1
别名 RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C8D3
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC002973
基因名称 RNASEH1
Gene ID (NCBI) 246243
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO60930
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].