验证数据展示
经过测试的应用
| Positive WB detected in | SK-N-SH cells, 4T1 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68787-3-Ig targets RNASEH1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RNASEH1 fusion protein Ag33948 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribonuclease H1 |
| 别名 | RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C8D3 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC002973 |
| 基因名称 | RNASEH1 |
| Gene ID (NCBI) | 246243 |
| RRID | AB_3670429 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O60930 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASEH1 antibody 68787-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |