验证数据展示

IF Staining of Retinal organoids using 15356-1-AP
Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.
WB analysis using 15356-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).
WB analysis using 15356-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 15356-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse eye using 15356-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Y79 using 15356-1-AP
Immunofluorescent analysis of (4% PFA) fixed Y79 cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of mouse eye using 15356-1-AP
IP result of anti-RLBP1 (IP:15356-1-AP, 4ug; Detection:15356-1-AP 1:3000) with mouse eye tissue lysate 2680 ug.
IF Staining of mouse eye using 15356-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 15356-1-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (19)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse eye tissue, HepG2 cells, mouse retina tissue, rat eye tissue
Positive IP detected in	mouse eye tissue
Positive IHC detected in	mouse eye tissue, human eye tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue, Retinal organoids
Positive IF/ICC detected in	HeLa cells, Y79 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 6 publications below
IHC	See 2 publications below
IF	See 13 publications below

产品信息

15356-1-AP targets RLBP1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, zebrafish
免疫原	CatNo: Ag7602 Product name: Recombinant human RLBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC004199 Sequence: MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRLQYPELFDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDENILPSDFGGTLPKYDGKAVAEQLFGPQAQAENTAF 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	retinaldehyde binding protein 1
别名	Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1
计算分子量	36 kDa
观测分子量	36 kDa
GenBank蛋白编号	BC004199
基因名称	RLBP1
Gene ID (NCBI)	6017
RRID	AB_2178530
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P12271
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

实验方案

Product Specific Protocols
WB protocol for RLBP1 antibody 15356-1-AP	Download protocol
IHC protocol for RLBP1 antibody 15356-1-AP	Download protocol
IF protocol for RLBP1 antibody 15356-1-AP	Download protocol
IP protocol for RLBP1 antibody 15356-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Adv Sci (Weinh) PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species Authors - Zhiyang Chen View Article
zebrafish	IF	Aging Cell Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina. Authors - Raquel R Martins View Article
human	WB,IF	Mol Vis Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients Authors - Michael H Farkas View Article
mouse	IF	Diabetes CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Authors - Jose-Andres C Portillo View Article
mouse	WB	FASEB J A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion. Authors - Jose-Andres C Portillo View Article
rat	IF	iScience Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration Authors - Haibin Tian View Article

验证数据展示

IF Staining of Retinal organoids using 15356-1-AP

WB analysis using 15356-1-AP

IHC staining of human eye using 15356-1-AP

WB analysis using 15356-1-AP

IF Staining of HeLa using 15356-1-AP

IF Staining of mouse eye using 15356-1-AP

IF Staining of Y79 using 15356-1-AP

IP experiment of mouse eye using 15356-1-AP

IF Staining of mouse eye using 15356-1-AP

IHC staining of mouse eye using 15356-1-AP

IHC staining of mouse eye using 15356-1-AP

RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species

Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.

Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients

CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.

A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion.

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration