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验证数据展示

  • IF Staining of Retinal organoids using 15356-1-AP

    IF Staining of Retinal organoids using 15356-1-AP

    Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • WB analysis using 15356-1-AP

    WB analysis using 15356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human eye using 15356-1-AP

    IHC staining of human eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RLBP1 antibody included
  • WB analysis using 15356-1-AP

    WB analysis using 15356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15356-1-AP

    IF Staining of HeLa using 15356-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 15356-1-AP

    IF Staining of mouse eye using 15356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Y79 using 15356-1-AP

    IF Staining of Y79 using 15356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse eye using 15356-1-AP

    IP experiment of mouse eye using 15356-1-AP

    IP result of anti-RLBP1 (IP:15356-1-AP, 4ug; Detection:15356-1-AP 1:3000) with mouse eye tissue lysate 2680 ug.

  • IF Staining of mouse eye using 15356-1-AP

    IF Staining of mouse eye using 15356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 15356-1-AP

    IHC staining of mouse eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RLBP1 antibody included
  • IHC staining of mouse eye using 15356-1-AP

    IHC staining of mouse eye using 15356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RLBP1 antibody included

RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 15356-1-AP

产品说明书

引用文献(25)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse eye tissue, HepG2 cells, mouse retina tissue, rat eye tissue
Positive IP detected inmouse eye tissue
Positive IHC detected inmouse eye tissue, human eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue, Retinal organoids
Positive IF/ICC detected inHeLa cells, Y79 cells
Planning an IHC experiment? We recommend our IHCeasy® RLBP1 Ready-To-Use IHC Kit. RLBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15356-1-AP targets RLBP1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, zebrafish
免疫原

CatNo: Ag7602

Product name: Recombinant human RLBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC004199

Sequence: MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRLQYPELFDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDENILPSDFGGTLPKYDGKAVAEQLFGPQAQAENTAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinaldehyde binding protein 1
别名 Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004199
基因名称 RLBP1
Gene ID (NCBI) 6017
RRIDAB_2178530
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12271
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

实验方案

Product Specific Protocols
IF protocol for RLBP1 antibody 15356-1-APDownload protocol
IHC protocol for RLBP1 antibody 15356-1-APDownload protocol
IP protocol for RLBP1 antibody 15356-1-APDownload protocol
WB protocol for RLBP1 antibody 15356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species

Authors - Zhiyang Chen
View Article
humanWB,IF

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
View Article
human,mouseIF

J Transl Med

Cargo molecules in plasma neural-derived extracellular vesicles reveal pathological signatures of primary open-angle glaucoma.

Authors - Xiang-Yuan Song
View Article
mouseIF

Diabetes

CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.

Authors - Jose-Andres C Portillo
View Article
humanIF

Stem Cell Res Ther

Determining the optimal stage for cryopreservation of human embryonic stem cell-derived retinal pigment epithelial cells

Authors - Ting Zhang
View Article
humanIF

Stem Cell Res Ther

The influence of femtosecond laser intrastromal lenticules on the characteristics and maturity in tissue-engineered stem cell-derived retinal pigment epithelium sheets

Authors - Gu Jianing
View Article
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