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RIP3 Polyclonal antibody

RIP3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29080-1-AP

Print datasheet

Synonyms

Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3

验证数据展示

  • WB analysis using 29080-1-AP

    WB analysis using 29080-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 29080-1-AP (RIP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy RIPK3 Ready-To-Use IHC Kit. RIPK3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29080-1-AP targets RIP3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen RIP3 fusion protein Ag30337 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name receptor-interacting serine-threonine kinase 3
Synonyms Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3
Calculated Molecular Weight 518 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC062584
Gene Symbol RIPK3
Gene ID (NCBI) 11035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIPK3,also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. RIPK3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus(PMID:18533105). It has 3 isoforms produced by alternative splicing. RIPK3 might form a homodimer within cells, and its apoptotic activity may not be required for this dimerization(PMID:18533105).

实验方案

Product Specific Protocols
WB protocol for RIP3 antibody 29080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols