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验证数据展示

  • WB analysis using 85760-4-RR

    WB analysis using 85760-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85760-4-RR (RIPK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RIPK2 Recombinant antibody

RIPK2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85760-4-RR

产品说明书

CloneNo. 250069D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

CARD3, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARDIAK, CCK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, THP-1 cells, Ramos cells, Raji cells, Jurkat cells, EL-4 cells
Planning an IHC experiment? We recommend our IHCeasy RIPK2 Ready-To-Use IHC Kit. RIPK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85760-4-RR targets RIPK2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 RIPK2 fusion protein Ag0909 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 receptor-interacting serine-threonine kinase 2
别名 CARD3, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARDIAK, CCK
计算分子量 61 kDa
观测分子量 46-55 kDa, 61-72 kDa
GenBank蛋白编号BC004553
基因名称 RIPK2
Gene ID (NCBI) 8767
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43353
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RIPK2 antibody 85760-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols