验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
29932-1-AP targets RIPK1 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | RIPK1 fusion protein Ag31950 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
别名 | EC:2.7.11.1, Receptor interacting serine/threonine protein kinase 1, Receptor-interacting protein 1, Receptor-interacting serine/threonine-protein kinase 1, RIP |
计算分子量 | 76KD |
观测分子量 | 70-80 kDa |
GenBank蛋白编号 | NM_003804 |
基因名称 | RIPK1 |
Gene ID (NCBI) | 8737 |
RRID | AB_3086193 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13546 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397).
实验方案
Product Specific Protocols | |
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WB protocol for RIPK1 antibody 29932-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Ethnopharmacol The protective role of Cordyceps cicadae and its active ingredient Myriocin against sodium iodate-induced age-related macular degeneration via an anti-necroptotic TNF-RIPK1/3 pathway | ||
Heliyon Identification of colon adenocarcinoma necroptosis subtypes and tumor antigens for the development of mRNA vaccines |