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验证数据展示

  • WB analysis of HepG2 using 14567-1-AP

    WB analysis of HepG2 using 14567-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14567-1-AP

    IHC staining of human breast cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 14567-1-AP

    IF Staining of HepG2 using 14567-1-AP

    Immunofluorescent analysis of HepG2 cells using 14567-1-AP (RINT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RINT1 Polyclonal antibody

RINT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14567-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

RINT-1, RINT 1, RAD50-interacting protein 1, HsRINT-1, HsRINT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14567-1-AP targets RINT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RINT1 fusion protein Ag6145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD50 interactor 1
别名 RINT-1, RINT 1, RAD50-interacting protein 1, HsRINT-1, HsRINT1
计算分子量 91 kDa
观测分子量 87 kDa
GenBank蛋白编号BC068483
基因名称 RINT1
Gene ID (NCBI) 60561
RRIDAB_2269421
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NUQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.

实验方案

Product Specific Protocols
WB protocol for RINT1 antibody 14567-1-APDownload protocol
IHC protocol for RINT1 antibody 14567-1-APDownload protocol
IF protocol for RINT1 antibody 14567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article
humanWB

Front Immunol

Circular RNA TAF4B targeting MFN2 accelerates cell growth in bladder cancer through p27 depression and AKT activation

Authors - Xiaoting Zhang
View Article