验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68469-1-Ig targets MIRO2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag29690 Product name: Recombinant human RHOT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 388-548 aa of BC014942 Sequence: LCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ras homolog gene family, member T2 |
| 别名 | RHOT2, 1A3C2, ARHT2, C16orf39, EC:3.6.5.- |
| 计算分子量 | 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC014942 |
| 基因名称 | RHOT2 |
| Gene ID (NCBI) | 89941 |
| RRID | AB_3085180 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8IXI1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RHOT2 (Mitochondrial Rho GTPase 2) is also named as MIRO-2, hMiro-2, ARHT2, C16orf39 and Ras homolog gene family member T2. RHOT2 belongs to the mitochondrial Rho GTPase family. RHOT2 gene encodes a member of Rho family of GTPase, which are localized to the outer membrane of mitochondria (PMID: 22078885).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIRO2 antibody 68469-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
