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验证数据展示

  • WB analysis using 68469-1-Ig

    WB analysis using 68469-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68469-1-Ig

    WB analysis using 68469-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

MIRO2 Monoclonal antibody

MIRO2 Monoclonal Antibody for WB, ELISA
Cat No. 68469-1-Ig

产品说明书

CloneNo. 1A3C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

RHOT2, 1A3C2, ARHT2, C16orf39, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68469-1-Ig targets MIRO2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MIRO2 fusion protein Ag29690 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ras homolog gene family, member T2
别名 RHOT2, 1A3C2, ARHT2, C16orf39, EC:3.6.5.-
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC014942
基因名称 RHOT2
Gene ID (NCBI) 89941
RRIDAB_3085180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IXI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RHOT2 (Mitochondrial Rho GTPase 2) is also named as MIRO-2, hMiro-2, ARHT2, C16orf39 and Ras homolog gene family member T2. RHOT2 belongs to the mitochondrial Rho GTPase family. RHOT2 gene encodes a member of Rho family of GTPase, which are localized to the outer membrane of mitochondria (PMID: 22078885).

实验方案

Product Specific Protocols
WB protocol for MIRO2 antibody 68469-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols