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验证数据展示

  • WB analysis of HEK-293 using 11949-1-AP

    WB analysis of HEK-293 using 11949-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 11949-1-AP

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 11949-1-AP

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

RHCG Polyclonal antibody

RHCG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11949-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (2)

别名

Tumor-related protein DRC2, RHGK, PDRC2, CDRC2, C15orf6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IHC detected inhuman kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11949-1-AP targets RHCG in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RHCG fusion protein Ag2493 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rh family, C glycoprotein
别名 Tumor-related protein DRC2, RHGK, PDRC2, CDRC2, C15orf6
计算分子量 479 aa, 53 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC030965
基因名称 RHCG
Gene ID (NCBI) 51458
RRIDAB_2269482
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBD6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHCG antibody 11949-1-APDownload protocol
IHC protocol for RHCG antibody 11949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,CoIP

Kidney Int

The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.

Authors - Soline Bourgeois
  • KO Validated
View Article
humanWB,IHC

Aging (Albany NY)

Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.

Authors - Wenguang Xu
View Article