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验证数据展示

  • WB analysis using 88102-1-RR

    WB analysis using 88102-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 88102-1-RR

    IF Staining of mouse cerebellum using 88102-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 88102-1-RR

    IF Staining of mouse cerebellum using 88102-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RGS8 Recombinant monoclonal antibody

RGS8 Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 88102-1-RR

产品说明书

CloneNo. 260122C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, ELISA

别名

Regulator of G-protein signaling 8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, pig brain tissue, rat liver tissue, rat brain tissue
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88102-1-RR targets RGS8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag26535

Product name: Recombinant human RGS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-198 aa of BC069718

Sequence: NKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 regulator of G-protein signaling 8
别名 Regulator of G-protein signaling 8
计算分子量 198 aa, 23 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC069718
基因名称 RGS8
Gene ID (NCBI) 85397
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.

实验方案

Product Specific Protocols
IF protocol for RGS8 antibody 88102-1-RRDownload protocol
WB protocol for RGS8 antibody 88102-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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