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验证数据展示

  • WB analysis using 88102-1-RR (same clone as 88102-1-PBS)

    WB analysis using 88102-1-RR (same clone as 88102-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03353-2

    Sandwich ELISA sample test of MP03353-2

    The mean RGS8 concentration was determined to be 88.79 ng/mL in mouse liver extract based on a 8.40 mg/mL extract load, 4.65 ng/mL in mouse skin extract based on a 0.90 mg/mL extract load and 41.22 ng/mL in rat brain extract based on a 3.90 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03353-2

    Sandwich ELISA standard curve of MP03353-2

    Sandwich ELISA standard curve of MP03353-2, Human RGS8 Recombinant Matched Antibody Pair - PBS only. 88102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26535. 88102-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

    IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

    IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

RGS8 Recombinant monoclonal antibody, PBS Only (Capture)

RGS8 Uni-rAb® Recombinant Antibody for WB, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 88102-1-PBS

产品说明书

CloneNo. 260122C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, Sandwich ELISA, Indirect ELISA

别名

Regulator of G-protein signaling 8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88102-1-PBS targets RGS8 as part of a matched antibody pair:

MP03353-2: 88102-1-PBS capture and 88102-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag26535

Product name: Recombinant human RGS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-198 aa of BC069718

Sequence: NKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 regulator of G-protein signaling 8
别名 Regulator of G-protein signaling 8
计算分子量 198 aa, 23 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC069718
基因名称 RGS8
Gene ID (NCBI) 85397
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57771
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.

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